Grönvik K O, Andersson J, Alm G, Stern P
Scand J Immunol. 1983 Oct;18(4):307-17. doi: 10.1111/j.1365-3083.1983.tb01802.x.
Lymphocytes from athymic and normal mice were tested and compared for cytotoxic activity after in vitro cultures supplemented with concanavalin A or T-cell growth factor (TCGF). Our results indicate that, in addition to cytotoxic T cells, natural killer (NK) activity can be recovered from lymphocytes cultured in the presence of TCGF and that this is not the result of contaminating interferon in the TCGF preparations. The NK nature of the effector cells was established by means of a panel of target cells, including a teratocarcinoma tumour cell, which enabled the distinction of T-cell- and NK-cell-mediated lysis.
在添加了刀豆球蛋白A或T细胞生长因子（TCGF）的体外培养后，对无胸腺小鼠和正常小鼠的淋巴细胞进行细胞毒性活性测试和比较。我们的结果表明，除了细胞毒性T细胞外，在TCGF存在下培养的淋巴细胞中可恢复自然杀伤（NK）活性，并且这不是TCGF制剂中污染干扰素的结果。通过一组靶细胞确定了效应细胞的NK性质，其中包括一个畸胎瘤肿瘤细胞，这使得能够区分T细胞和NK细胞介导的裂解。
