Skinner M, Marbrook J
Aust J Exp Biol Med Sci. 1983 Aug;61 (Pt 4):461-70. doi: 10.1038/icb.1983.43.
Subsets of natural killer cells may be characterised by the target cell profile, cell surface markers and the effect of growth factors on the production of effector cells. The subset which appears in primary cultures (spontaneous or natural T killer cells) is enhanced by the supernatants of Con A-stimulated rat spleen cultures. The enhancement can be attributed to an increased number of clones rather than an expansion of clone size in 5-day cultures. With semi-purified IL-2 preparations, clones of spontaneous cytotoxic cells are also detectable in nu/nu spleen cultures. These results suggest such spontaneous cytotoxicity is a general phenomenon within cytotoxic responses.
自然杀伤细胞的亚群可通过靶细胞谱、细胞表面标志物以及生长因子对效应细胞产生的影响来进行表征。出现在原代培养物中的亚群（自发或天然T杀伤细胞）会被伴刀豆球蛋白A刺激的大鼠脾脏培养物的上清液增强。这种增强可归因于克隆数量的增加，而非5天培养物中克隆大小的扩大。使用半纯化的白细胞介素-2制剂，在无胸腺裸鼠脾脏培养物中也可检测到自发细胞毒性细胞的克隆。这些结果表明，这种自发细胞毒性是细胞毒性反应中的一种普遍现象。
