人T细胞生长因子的分离与部分特性鉴定

Isolation and partial characterization of human T-cell growth factor.

作者信息

Böhlen P, Esch F, Wegemer D, Salk P, Dennert G

出版信息

Biochem Biophys Res Commun. 1983 Dec 16;117(2):623-30. doi: 10.1016/0006-291x(83)91246-9.

Abstract

Human T-cell growth factor (TCGF) has been isolated from conditioned media of the Jurkat T-leukemia cell line. Using a high-efficiency isolation procedure involving hollow fiber concentration, gel filtration and 3 steps of reverse-phase HPLC we obtained 100 to 600 pmol TCGF per liter of conditioned medium. Jurkat cell-derived TCGF (jTCGF) has a molecular weight of 15,750. The amino acid composition of jTCGF agrees well with that derived from the cDNA sequence coding for this protein (Taniguchi et al, Nature 302, 305, 1983). jTCGF is highly active in vitro in stimulating the proliferation of T-cells as measured by 3H-thymidine incorporation into DNA (half-maximal stimulation with 3 fmol/100 microliters well).

摘要

人T细胞生长因子（TCGF）已从Jurkat T白血病细胞系的条件培养基中分离出来。采用包括中空纤维浓缩、凝胶过滤和三步反相高效液相色谱在内的高效分离程序，我们从每升条件培养基中获得了100至600皮摩尔的TCGF。源自Jurkat细胞的TCGF（jTCGF）分子量为15750。jTCGF的氨基酸组成与编码该蛋白的cDNA序列推导的氨基酸组成非常吻合（谷口等，《自然》302, 305, 1983）。通过将3H-胸腺嘧啶核苷掺入DNA来测量，jTCGF在体外刺激T细胞增殖方面具有高活性（每孔3飞摩尔/100微升时达到最大刺激的一半）。