Clark S C, Arya S K, Wong-Staal F, Matsumoto-Kobayashi M, Kay R M, Kaufman R J, Brown E L, Shoemaker C, Copeland T, Oroszlan S
Proc Natl Acad Sci U S A. 1984 Apr;81(8):2543-7. doi: 10.1073/pnas.81.8.2543.
The partial amino acid sequences of human T-cell growth factors (TCGFs) isolated from normal peripheral blood lymphocytes and from a leukemia T-cell line (Jurkat) show that the amino-terminal sequences of the two proteins (15 residues) are identical. Oligonucleotides based on the published Jurkat TCGF DNA sequence were used to isolate six cDNA clones of TCGF mRNA from normal lymphocytes. The predicted amino acid sequence of normal lymphocyte TCGF was identical to the sequence of the Jurkat protein, showing that the differences in biochemical properties of the two proteins result from post-translational events. Amino acid and nucleotide sequence data suggest that TCGF is derived from a precursor polypeptide that is cleaved at the amino terminus but not at the carboxyl terminus. Hybridization of the cloned lymphocyte TCGF cDNA to cellular DNA and RNA strongly suggested that the TCGF gene is expressed as a single mRNA species from a single-copy gene. No differences in the organization of the TCGF gene in normal, leukemic, and human T-cell leukemia/lymphoma virus-infected cells was detected regardless of whether they produce TCGF or not.
从正常外周血淋巴细胞和白血病T细胞系（Jurkat）中分离出的人T细胞生长因子（TCGF）的部分氨基酸序列表明，这两种蛋白质的氨基末端序列（15个残基）是相同的。基于已发表的Jurkat TCGF DNA序列合成的寡核苷酸，被用于从正常淋巴细胞中分离出六个TCGF mRNA的cDNA克隆。正常淋巴细胞TCGF的预测氨基酸序列与Jurkat蛋白的序列相同，这表明这两种蛋白质生化特性的差异是由翻译后事件导致的。氨基酸和核苷酸序列数据表明，TCGF来源于一种前体多肽，该前体多肽在氨基末端被切割，但在羧基末端未被切割。克隆的淋巴细胞TCGF cDNA与细胞DNA和RNA的杂交结果强烈表明，TCGF基因以单拷贝基因表达为单一的mRNA种类。无论正常、白血病以及感染人T细胞白血病/淋巴瘤病毒的细胞是否产生TCGF，均未检测到TCGF基因在组织上存在差异。
