Mikhaĭlova A T, Gorgoliuk N A, Virtanen I, Lehto V P
Ontogenez. 1984 Mar-Apr;15(2):137-45.
The water extracts from the retina and brain of 7-8-day old chick embryos were centrifuged at 20,000 g; sediments were discarded and supernatants were additionally centrifuged at 110,000 g. The inductive activity of supernatants (20,000 and 110,000 g) and sediments (110,000 g) was estimated in vitro on the Rana temporaria early gastrula ectoderm. The neutralizing activity was related exclusively to the soluble fractions of the extracts from the chick embryo retina and brain. The lens-inducing activity appeared to be characteristic of both the supernatants and the microsome fractions of these extracts. A comparative biochemical analysis of the extracts (isoelectrofocusing, electrophoresis in the presence of sodium dodecylsulfate, electroblotting) has shown that the chick embryo retina and brain are similar by the spectrum and properties of peptides. It is suggested that the similarity of the extracts inducing effect on the early gastrula ectoderm is due to the presence of the same proteins (peptides) in the retina and brain. Peptides with a positive immunohistochemical reaction to vimentin and peptides of neurofilaments were found in trace quantities in the retina and brain extracts by means of immunoelectroblotting.
将7-8日龄鸡胚的视网膜和脑的水提取物在20,000 g下离心；弃去沉淀物，上清液再在110,000 g下离心。在林蛙早期原肠胚外胚层上体外评估上清液（20,000和110,000 g）和沉淀物（110,000 g）的诱导活性。中和活性仅与鸡胚视网膜和脑提取物的可溶部分有关。晶状体诱导活性似乎是这些提取物的上清液和微粒体部分所特有的。对提取物进行的比较生化分析（等电聚焦、十二烷基硫酸钠存在下的电泳、电印迹）表明，鸡胚视网膜和脑在肽的光谱和性质方面相似。有人认为，提取物对早期原肠胚外胚层诱导作用的相似性是由于视网膜和脑中存在相同的蛋白质（肽）。通过免疫电印迹法在视网膜和脑提取物中发现了对波形蛋白呈阳性免疫组化反应的肽和神经丝肽，但含量极少。
