Ellis D, Buffone G J
Clin Chem. 1977;23(4):666-70.
We evaluated the use of immunonephelometric methods for measuring specific urinary proteins. Using a nephelometer to detect light scattering (angle, 31 degrees), we measured some proteins immunonephelometrically in serum and aliquots of 24-h urines from 50 apparently healthy children, ages 2-17 years. The mean urinary excretion rate (mg/24h) and the range of values was: for albumin 5.5 (range, 0-13.3), for transferrin 0.5 (0-1.9, for IgG 3.3 0-12), and for alpha 2-macroglobulin 0.6 (0-2.3). Direct comparison of the values for pathological urines with those for a reference population may offer more meaningful information concerning the integrity of the glomerular basement membrane than is provided by protein selectivity indices, and measuring a plasma protein such as albumin in urine may better define pathological proteinuria.
我们评估了免疫比浊法在测量特定尿蛋白中的应用。使用比浊仪检测光散射（角度为31度），我们对50名年龄在2至17岁的看似健康儿童的血清和24小时尿液样本进行了免疫比浊法蛋白质测量。尿排泄率均值（mg/24小时）及数值范围如下：白蛋白为5.5（范围0 - 13.3），转铁蛋白为0.5（0 - 1.9），免疫球蛋白G为3.3（0 - 12），α2 - 巨球蛋白为0.6（0 - 2.3）。将病理性尿液的值与参考人群的值直接比较，可能会比蛋白质选择性指数提供更多有关肾小球基底膜完整性的有意义信息，并且测量尿液中的血浆蛋白如白蛋白可能会更好地界定病理性蛋白尿。
