Jennings P A, Finch J T, Winter G, Robertson J S
Cell. 1983 Sep;34(2):619-27. doi: 10.1016/0092-8674(83)90394-x.
Subgenomic RNAs (sgRNAs) were isolated from defective interfering virus produced by high multiplicity passage of the human influenza strain A/PR/8/34. Cloning and sequencing of 35 unique sgRNAs revealed that many were about 400 nucleotides long, containing about 200 nucleotides from each of the 5' and 3' ends of a full-length segment. Most of the sgRNAs were derived from segment 1, but there were examples from six other segments, including those encoding the haemagglutinin and neuraminidase. Our analysis of the sequence rearrangements found in sgRNAs indicates that they may be generated from the standard viral segments by a jumping viral polymerase that makes transitions between adjacent regions of the RNA template in the ribonucleoprotein tertiary structure.
亚基因组RNA（sgRNAs）是从人甲型流感病毒株A/PR/8/34高倍传代产生的缺陷干扰病毒中分离得到的。对35种独特的sgRNAs进行克隆和测序后发现，许多sgRNAs长度约为400个核苷酸，从全长片段的5'端和3'端各包含约200个核苷酸。大多数sgRNAs来源于片段1，但也有来自其他六个片段的例子，包括编码血凝素和神经氨酸酶的片段。我们对sgRNAs中发现的序列重排的分析表明，它们可能是由一种跳跃的病毒聚合酶从标准病毒片段产生的，这种聚合酶在核糖核蛋白三级结构的RNA模板的相邻区域之间进行转换。
