来自甲型流感病毒A/PR/8/34的两种亚基因组RNA的结构
The structure of two subgenomic RNAs from human influenza virus A/PR/8/34.
作者信息
Winter G, Fields S, Ratti G
出版信息
Nucleic Acids Res. 1981 Dec 21;9(24):6907-15. doi: 10.1093/nar/9.24.6907.
Abstract
The nucleotide sequences of two subgenomic RNA segments from influenza virus A/PR/8/34 have been determined by cloning viral cDNA into the vector M13mp7. Sequence analysis was facilitated by a re-cloning strategy which takes advantage of both wild-type and amber derivatives of the M13 vector. The RNA species (444 and 480 nucleotides) contain the 5' and 3' termini of segment 1 and therefore derive by simple internal deletions of this segment. However, these species are not exact copies of the terminal regions of the progenitor segment but contain a few base changes. These differences suggest that after these RNAs have arisen, their sequences can drift, presumably reflecting a lower selective pressure than on the standard RNA segments.
摘要
通过将甲型流感病毒A/PR/8/34的病毒cDNA克隆到载体M13mp7中，测定了该病毒两个亚基因组RNA片段的核苷酸序列。一种重新克隆策略促进了序列分析，该策略利用了M13载体的野生型和琥珀突变衍生物。这两种RNA片段（分别为444和480个核苷酸）包含第1节段的5'和3'末端，因此是该节段通过简单的内部缺失产生的。然而，这些片段并非原始节段末端区域的精确拷贝，而是包含一些碱基变化。这些差异表明，这些RNA产生后，其序列可能会发生漂移，大概反映出与标准RNA片段相比更低的选择压力。
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