Zimakov Iu A, Gukasov V M, Kaplan E Ia, Zimakova I E
Farmakol Toksikol. 1983 Jul-Aug;46(4):20-3.
Use was made of a model system initiating lipid peroxidation, that contained phosphate buffer, H2O2 and eosine. Chemiluminescence of the system decreased after addition to rat brain synaptosomes, thereby indicating the presence of antioxidants in the animals' brain. After addition of brain synaptosomes of rats poisoned with high lethal doses of armine (0.4--0.5 mg/kg) a prooxidant effect was observed. It was manifested in the elimination of the effect of natural antioxidants contained by synaptosomes of normal rats. Addition of acetylcholine, armine, DFP and oxazyl at concentrations of 1.10(-4) 1.10(-3) M to the model system containing rat brain synaptosomes entailed a considerable intensification of chemiluminescence. Atropine and scopolamine did not produce any substantial effect on chemiluminescence, whereas dipiroxim exerted an overt antioxidant effect. The conclusion is made about the possibility of activating the system of lipid peroxidation as one of the components of the toxic action of anticholinesterase agents.
利用了一种引发脂质过氧化的模型系统，该系统包含磷酸盐缓冲液、过氧化氢和曙红。向大鼠脑突触体中添加该系统后，其化学发光减弱，从而表明动物脑中存在抗氧化剂。向用高致死剂量的胺（0.4 - 0.5毫克/千克）中毒的大鼠脑突触体中添加后，观察到促氧化作用。其表现为消除了正常大鼠突触体中所含天然抗氧化剂的作用。向含有大鼠脑突触体的模型系统中添加浓度为1.10(-4) 1.10(-3) M的乙酰胆碱、胺、二异丙基氟磷酸酯和恶唑，会使化学发光显著增强。阿托品和东莨菪碱对化学发光没有产生任何实质性影响，而双吡啶肟则表现出明显的抗氧化作用。得出结论：作为抗胆碱酯酶剂毒性作用的组成部分之一，脂质过氧化系统有可能被激活。
