Stocker J W
Immunology. 1977 Mar;32(3):283-90.
A two-stage tissue culture system was used to test the concept of clonal abortion as a mechanism for tolerance induction in B cells. In the first stage, neonatal spleen cells or bone marrow cells were cultured for 72 h under conditions in which B-cell neogenesis occurred. Haptens coupled to various carriers, were introduced during this stage. Following this culture phase, the cells were washed and their competence to respond to hapten-POL was measured in microcultures where feedback effects were minimized. The results indicated that immature B cells were specially susceptible to tolerance but that the conditions under which hapten was presented were also important in determining the outcome of the cell-antigen encounter.
采用两阶段组织培养系统来测试克隆流产这一概念,它是B细胞耐受诱导的一种机制。在第一阶段,将新生期脾细胞或骨髓细胞在发生B细胞新生的条件下培养72小时。在此阶段引入与各种载体偶联的半抗原。经过这个培养阶段后,洗涤细胞,并在微培养中测量它们对半抗原-POL反应的能力,在微培养中反馈效应被最小化。结果表明,未成熟B细胞特别容易发生耐受,但呈现半抗原的条件对于决定细胞与抗原相遇的结果也很重要。