Stocker J W
Immunology. 1977 Mar;32(3):275-81.
Maturation of B-cell function was studied in a two-stage tissue culture system. In the first stage, cells were cultured in the absence of antigen and then transferred to microcultures where the frequency of hapten-specific plaque-forming cell (PFC) precursors was determined; Bone-marrow cells and spleen cells from 6--8-day-old mice mice were shown to act as sources of B-cell neogenesis in vitro. Both populations had very low initial frequencies of hapten-specific PFC precursors, but this increased ten- to seventeen-fold during a period of 72 h in the preliminary cultures. This increase could not be accounted for by selective cell death, nor by decay of a suppressor cell subpopulation nor by proliferation of pre-existing Fc-receptor-bearing B cells. The mechanism for the increase in frequency of functional B cells in cultures of bone marrow and neonatal spleen was thus the result of maturation of B-cell precursors to a state of immune competence during the culture interval.
在一个两阶段组织培养系统中研究了B细胞功能的成熟情况。在第一阶段，细胞在无抗原的情况下培养，然后转移至微量培养物中，在其中测定半抗原特异性空斑形成细胞（PFC）前体的频率；结果显示，6至8日龄小鼠的骨髓细胞和脾细胞可作为体外B细胞新生的来源。这两种细胞群体的半抗原特异性PFC前体初始频率都非常低，但在初步培养的72小时内，该频率增加了10至17倍。这种增加不能用选择性细胞死亡来解释，也不能用抑制性细胞亚群的衰退或预先存在的带有Fc受体的B细胞的增殖来解释。因此，骨髓和新生脾细胞培养物中功能性B细胞频率增加的机制是B细胞前体在培养间隔期间成熟为免疫活性状态的结果。
