Peichl L, Wässle H
J Physiol. 1983 Aug;341:309-24. doi: 10.1113/jphysiol.1983.sp014807.
The correlation between the receptive field centre and the dendritic tree of individual brisk transient, or alpha, ganglion cells in the cat retina was investigated by a combination of physiological and anatomical techniques. The sizes of receptive field centres of brisk transient (Y) cells were measured with a flickering spot of light. Contour maps and response (or sensitivity) profiles were measured at mesopic and scotopic backgrounds. Recording positions on the retina and nearby blood vessels were back-projected onto the receptive field plots on the tangent screen. After recording, whole amount preparations of the retinae were stained by a reduced silver method to stain all alpha cells together with their dendritic trees. By comparing the landmarks on the screen plot with those of the whole mount it was possible to identify the recorded cells in the preparation and to study their morphology. The dendritic tree of an alpha cell determines the position, size and shape of its receptive field centre. The mesopic receptive field centres were found to be a factor of 1.4 +/- 0.13 larger than their respective dendritic fields. It is suggested that the dendritic fields of presynaptic neurones (bipolar and amacrine cell processes) add to the ganglion cell dendritic tree to produce the larger centre summating area.
通过生理学和解剖学技术相结合的方法，研究了猫视网膜中单个瞬态快适应（即α）神经节细胞的感受野中心与树突树之间的相关性。用闪烁的光点测量瞬态快适应（Y）细胞感受野中心的大小。在中间视觉和暗视觉背景下测量等高线图和反应（或灵敏度）剖面图。将视网膜上的记录位置和附近的血管反向投影到切线屏上的感受野图上。记录完成后，用还原银法对视网膜全量标本进行染色，以使所有α细胞及其树突树一起被染色。通过比较屏上图像的标记与整装标本的标记，可以在标本中识别出记录的细胞并研究其形态。α细胞的树突树决定了其感受野中心的位置、大小和形状。发现中间视觉感受野中心比其各自的树突野大1.4±0.13倍。有人提出，突触前神经元（双极细胞和无长突细胞突起）的树突野与神经节细胞的树突树相加，从而产生更大的中心总和区域。
