Dalen A B, Hellgren L, Iversen O J, Vincent J
Acta Pathol Microbiol Immunol Scand B. 1983 Aug;91(4):221-9. doi: 10.1111/j.1699-0463.1983.tb00037.x.
Virus-like particles were demonstrated by electron microscopy of specimens from psoriatic plaques. Cell cultures established from plaques released particles, showing the morphological features of a retrovirus, for a prolonged period. The particles had a buoyant density of 1.17 g/cc and contained a surface protein of Mr 70,000 which bound to concanavalin A and therefore probably was of glycoprotein nature. A major internal protein of Mr 30,000 and two minor proteins of Mr 18,000 and 15,000 were detected. The composition of the proteins therefore resembled that of murine C-type retrovirus. Patients with psoriasis release virus-like particles in their urine which have a different morphology to the particles from cell cultures. A similar difference could be observed in particles from cell cultures after treatment with normal urine. Patients with psoriatic arthritis had serum immune complexes. The complexes contained distorted particles which could not be identified as virions on a morphological basis. A radio immunoassay was performed which demonstrated common antigens in the virus-like particles isolated from psoriatic urines, immune complexes and cell cultures.
通过对银屑病斑块标本进行电子显微镜检查，发现了病毒样颗粒。从斑块建立的细胞培养物在较长时间内释放出具有逆转录病毒形态特征的颗粒。这些颗粒的浮力密度为1.17 g/cc，含有一种分子量为70,000的表面蛋白，该蛋白与伴刀豆球蛋白A结合，因此可能具有糖蛋白性质。检测到一种分子量为30,000的主要内部蛋白以及两种分子量分别为18,000和15,000的次要蛋白。因此，这些蛋白质的组成类似于鼠C型逆转录病毒。银屑病患者尿液中释放出的病毒样颗粒与细胞培养物中的颗粒形态不同。在用正常尿液处理后的细胞培养物颗粒中也可观察到类似差异。银屑病关节炎患者有血清免疫复合物。这些复合物含有形态上无法鉴定为病毒体的变形颗粒。进行了放射免疫测定，结果表明从银屑病尿液、免疫复合物和细胞培养物中分离出的病毒样颗粒存在共同抗原。
