Ruddick J H, Leslie G A
J Immunol. 1977 Mar;118(3):1025-31.
Chicken anti-human IgD antiserum (anti-delta) has demonstrated an antigenically cross-reactive homologue on rat lymphocytes. IgD and IgM are the only cell surface immunoglobulins detectable by the lactoperoxidase radiolabeling technique employed. The results indicate that, although rat surface IgD is antigenically distinct from rat IgM, the respective H chains co-electrophorese in 10% polyacrylamide-SDS gels. Rat delta-chain has an apparent m.w. of 73,000 daltons and exhibits a minor 65,000 dalton component which probably represents a partially degraded delta-chain. The ontogenic emergence of rat IgD occurs approximately 3.5 weeks after birth whereas IgM, in contrast, is apparent by 6 days of age. Thus, as in the human, IgM develops before IgD. IgD receptors are undetectable in the thymus but are present in increasing levels in spleen, blood, lymph nodes, and Peyer's patches.
鸡抗人IgD抗血清（抗δ）已在大鼠淋巴细胞上显示出一种抗原交叉反应性同源物。IgD和IgM是采用乳过氧化物酶放射性标记技术可检测到的仅有的细胞表面免疫球蛋白。结果表明，虽然大鼠表面IgD在抗原性上与大鼠IgM不同，但各自的重链在10%聚丙烯酰胺-SDS凝胶中共同电泳。大鼠δ链的表观分子量为73,000道尔顿，并呈现出一个较小的65,000道尔顿的组分，这可能代表部分降解的δ链。大鼠IgD的个体发生出现在出生后约3.5周，而相比之下，IgM在6日龄时就很明显。因此，与人类一样，IgM在IgD之前发育。在胸腺中检测不到IgD受体，但在脾脏、血液、淋巴结和派伊尔结中的水平不断升高。
