Geisler N, Plessmann U, Weber K
FEBS Lett. 1983 Oct 31;163(1):22-4. doi: 10.1016/0014-5793(83)81153-3.
The amino-terminal 98 residues of porcine vimentin have been determined by amino acid sequence studies. Extensive overlap is seen with the corresponding region of the carboxyterminal 448 residues of hamster vimentin predicted from DNA sequence studies, which left the very amino-terminal region unknown. The combined data show that contrary to gel electrophoretic results, mammalian vimentin contains only about 467 residues, and that species-specific drift occurs mainly in the amino-terminal non-alpha-helical array. The results are discussed parallel to emerging concepts on intermediate filament protein diversity.
通过氨基酸序列研究确定了猪波形蛋白氨基末端的98个残基。从DNA序列研究预测的仓鼠波形蛋白羧基末端448个残基的相应区域存在广泛重叠，而该区域的最氨基末端部分尚不清楚。综合数据表明，与凝胶电泳结果相反，哺乳动物波形蛋白仅含有约467个残基，并且物种特异性漂移主要发生在氨基末端非α螺旋序列中。结合中间丝蛋白多样性的新观点对这些结果进行了讨论。
