Gill S J, Skold R, Fall L, Shaeffer T, Spokane P, Wyman J
Science. 1978 Jul 28;201(4353):362-4. doi: 10.1126/science.663663.
Deoxygenation of concentrated solutions (0.33 gram per milliliter) of sickle cell hemoglobin show (i) a "crisis point" where the oxygen binding curve is unusually steep (Hill coefficient of 5 to 6), and (ii) a simultaneous increase in light scattering. Nearly identical oxygen binding curves are obtained upon oxygenation and deoxygenation of these solutions. The influence of aggregation is to shift the curve toward higher pressures.
镰状细胞血红蛋白浓缩溶液（0.33克/毫升）的脱氧显示出：（i）一个“危机点”，此时氧结合曲线异常陡峭（希尔系数为5至6），以及（ii）光散射同时增加。对这些溶液进行氧合和脱氧时可获得几乎相同的氧结合曲线。聚集的影响是使曲线向更高压力方向移动。
