Merlin D, Bucher U
Vox Sang. 1983;45(4):326-32. doi: 10.1111/j.1423-0410.1983.tb01922.x.
Two methods of preparation of platelet concentrates (PC) derived from citrate-phosphate-dextrose (CPD) whole blood have been compared: (1) resuspension after having left the PC undisturbed at room temperature for 1 h (according to Mourad), and (2) immediate resuspension of the PC after the centrifugation of a platelet-rich plasma which has been acidified beforehand by the addition of ACD. In vitro platelet yield in acidified (CPD/ACD-)PC was at least equal to and, in cases with a particularly strong postcentrifugal tendency for clumping of platelets, clearly better (p less than 0.05) than in the 'Mourad platelets'. The results show that it is possible to produce PC from fresh CPD whole blood without delay. This may be helpful in clinical situations where freshly prepared PC should be available immediately. A special double bag (Fenwal DFX 733) containing CPD-A in the primary bag and 10 ml of ACD in the satellite bag, allows preparation of PC under these conditions in a closed system.
比较了两种从枸橼酸盐 - 磷酸盐 - 葡萄糖（CPD）全血制备血小板浓缩物（PC）的方法：（1）在室温下将PC静置1小时后再重悬（根据穆拉德法），以及（2）对预先加入ACD酸化的富血小板血浆进行离心后立即将PC重悬。酸化（CPD/ACD-）PC的体外血小板产量至少与“穆拉德血小板”相当，在血小板离心后聚集倾向特别强的情况下，明显优于“穆拉德血小板”（p<0.05）。结果表明，可以毫不延迟地从新鲜CPD全血中制备PC。这在需要立即获得新鲜制备的PC的临床情况下可能会有帮助。一种特殊的双层袋（芬瓦尔DFX 733），主袋中含有CPD-A，卫星袋中含有10毫升ACD，可在这些条件下在封闭系统中制备PC。
