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血小板浓缩物的制备与储存。I. 影响从全血中收获存活血小板的因素。

Preparation and storage of platelet concentrates. I. Factors influencing the harvest of viable platelets from whole blood.

作者信息

Slichter S J, Harker L A

出版信息

Br J Haematol. 1976 Nov;34(3):395-402. doi: 10.1111/j.1365-2141.1976.tb03586.x.

DOI:10.1111/j.1365-2141.1976.tb03586.x
PMID:990181
Abstract

Factors affecting the yield and viability of concentrated platelets have been investigated in a blood component programme. It was found that 86+/-1% of the platelets from a unit of whole blood can be concentrated without loss of viability by processing ACD or CPD anticoagulated blood at room temperature. The steps are initial centrifugation at 1000 g for 9 min to harvest platelet-rich plasma; centrifugation of the platelet-rich plasma at 3000 g for 20 min to pack the platelets; and resuspension of the platelet pellet in residual plasma after 1 1/2 h.

摘要

在一个血液成分项目中,对影响浓缩血小板产量和活力的因素进行了研究。结果发现,通过在室温下处理用ACD或CPD抗凝的全血,一个单位全血中86±1%的血小板能够被浓缩且活力不丧失。步骤如下:首先以1000g离心9分钟以收集富含血小板的血浆;然后将富含血小板的血浆以3000g离心20分钟以包装血小板;最后在1个半小时后将血小板沉淀重悬于残余血浆中。

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Preparation and storage of platelet concentrates. I. Factors influencing the harvest of viable platelets from whole blood.血小板浓缩物的制备与储存。I. 影响从全血中收获存活血小板的因素。
Br J Haematol. 1976 Nov;34(3):395-402. doi: 10.1111/j.1365-2141.1976.tb03586.x.
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