豚鼠结肠带平滑肌的磷-31核磁共振研究。

Phosphorus-31 NMR studies of smooth muscle from guinea-pig taenia coli.

作者信息

Vogel H J, Lilja H, Hellstrand P

出版信息

Biosci Rep. 1983 Sep;3(9):863-70. doi: 10.1007/BF01133785.

Abstract

Phosphorus-31 NMR spectra of superfused isometrically mounted guinea-pig taenia coli were obtained using a horizontal probe at 103.2 MHz. The spectra showed resonances for ATP, phosphocreatine (PCr), and a sugar phosphate resonance. The PCr/ATP ratio was between 1.5 and 2.0 consistent with chemical analysis of tissue extracts. The level of PCr, but not of ATP, decreases reversibly during contraction or inhibition of respiration. These conditions did not cause substantial changes in the intracellular pH, which was 7.0 +/- 0.1.

摘要

使用水平探头在103.2兆赫频率下获得了等长收缩的豚鼠结肠带绦虫超融合状态下的磷-31核磁共振谱。该谱显示了三磷酸腺苷（ATP）、磷酸肌酸（PCr）的共振峰以及一个磷酸糖共振峰。PCr/ATP比值在1.5至2.0之间，与组织提取物的化学分析结果一致。在收缩或呼吸抑制过程中，PCr水平可逆性降低，但ATP水平未变。这些情况并未导致细胞内pH值发生显著变化，细胞内pH值为7.0±0.1。