Nishikawa H, Fujii T, Yamada S, Yoshizaki K, Watari H
J Biochem. 1980 Feb;87(2):663-6. doi: 10.1093/oxfordjournals.jbchem.a132791.
31P-NMR measurements of brain slices of guinea pig were successfully carried out for the first time with a new perfusion and spinning technique. The 31P-NMR spectrum showed resonance lines of phosphocreatine, fructose-6-phosphate, ATP, and inorganic phosphate. In the time course of the 31P-NMR spectra, the concentrations of phosphocreatine and fructose-6-phosphate, which were estimated from the initial spectrum, were 4.3 and 1.1 mumol/g of fresh tissue, respectively, and the estimates from the following ones showed that the concentration of phosphocreatine decreased to 2.7 mumol/g of fresh tissue while that of fructose-6-phosphate did not change significantly throughout the measurements.
首次使用一种新的灌注和旋转技术成功地对豚鼠脑切片进行了³¹P-NMR测量。³¹P-NMR光谱显示了磷酸肌酸、6-磷酸果糖、ATP和无机磷酸盐的共振线。在³¹P-NMR光谱的时间进程中，根据初始光谱估计的磷酸肌酸和6-磷酸果糖的浓度分别为4.3和1.1μmol/g新鲜组织，而随后的估计表明，在整个测量过程中，磷酸肌酸的浓度降至2.7μmol/g新鲜组织，而6-磷酸果糖的浓度没有显著变化。
