McClung H J, Powers P A, Sloan H R, Kerzner B
Clin Chim Acta. 1983 Nov 15;134(3):245-54. doi: 10.1016/0009-8981(83)90364-9.
We describe a gas-liquid chromatographic technique for quantifying the low molecular weight (Mr 106-634) oligomers of polyethylene glycol (PEG) in clinical specimens. The deionized sample, containing tetra-ethylene glycol as an internal standard, is applied on column. This technique readily quantifies as little as 2.5 micrograms of an individual oligomer; with such a quantity, the coefficient of variation is +/- 2.5 percent (N = 25 analyses). Small volumes (250 microliter) of urine are conveniently analyzed, and a single column can be utilized for the analysis of approximately three hundred specimens. We have analyzed timed urine specimens from humans who received 0.15 g of PEG 400 per kilogram. Individuals varied markedly with regard to the total amount of PEG excreted into the urine; each subject, however, consistently excretes a uniform percentage of the ingested dose. The urinary oligomeric profile of PEG does not vary from subject to subject nor from hour to hour, during the first six hours following oral administration, so that a random urine obtained during this period provides a reliable clinical specimen. This technique should facilitate clinical studies that utilize polyethylene glycol 400 as an index of passive intestinal transport.
我们描述了一种气相色谱技术，用于定量临床样本中聚乙二醇（PEG）的低分子量（Mr 106 - 634）低聚物。将含有四甘醇作为内标的去离子样品注入色谱柱。该技术能够轻松定量低至2.5微克的单个低聚物；对于这样的量，变异系数为±2.5%（N = 25次分析）。小体积（250微升）的尿液便于分析，一根色谱柱可用于分析约三百个样本。我们分析了每千克体重接受0.15克PEG 400的人的定时尿液样本。个体在尿液中排出的PEG总量差异显著；然而，每个受试者始终以均匀的百分比排出摄入剂量。在口服给药后的前六个小时内，PEG的尿低聚物谱在个体之间以及不同时间之间没有变化，因此在此期间获得的随机尿液提供了可靠的临床样本。该技术应有助于利用聚乙二醇400作为被动肠道转运指标的临床研究。
