Carper W R, Campbell D P, Morrical S W, Thompson R E
Experientia. 1983 Nov 15;39(11):1295-7. doi: 10.1007/BF01990378.
Porcine liver beta-D-glucose dehydrogenase has been isolated using Triton X-114 to release it from the endoplasmic reticulum. The purified enzyme contains a limited amount (1.7%) of lipid material, including cholesterol, fatty acids, mono and diglycerides, phosphatidylcholine, phosphatidylethanolamine, and cholesterol esters. This enzyme is a tetrameric protein containing an extensive number of hydrophobic residues. This form of glucose dehydrogenase is capable of turning over both beta-D-glucose and alpha-D-glucose-6-phosphate in vivo as indicated from a steady state kinetic analysis at 37 degrees C.
使用Triton X-114从内质网中释放猪肝脏β-D-葡萄糖脱氢酶，从而将其分离出来。纯化后的酶含有少量（1.7%）的脂质物质，包括胆固醇、脂肪酸、甘油单酯和甘油二酯、磷脂酰胆碱、磷脂酰乙醇胺以及胆固醇酯。这种酶是一种四聚体蛋白，含有大量疏水残基。如在37℃下进行的稳态动力学分析所示，这种形式的葡萄糖脱氢酶在体内能够催化β-D-葡萄糖和α-D-葡萄糖-6-磷酸。
