Cheetham R C, Fletcher S M, Whitehead P H
Forensic Sci Int. 1983 Aug-Sep;22(2-3):195-201. doi: 10.1016/0379-0738(83)90014-2.
A simple method for the extraction of testosterone from bloodstains followed by its measurement by radioimmunoassay is described. Complete discrimination of males and females was achieved with measured bloodstains as small as 40 microliters. With stains of unknown volume the total protein content of the stain was determined as an internal reference level. Using the testosterone/protein ratio unequivocal identification was possible for 75% of the stains from males and 50% from females.
描述了一种从血迹中提取睾酮并随后通过放射免疫测定法进行测量的简单方法。对于小至40微升的被测血迹，实现了对男性和女性的完全区分。对于体积未知的血迹，测定血迹中的总蛋白含量作为内部参考水平。利用睾酮/蛋白比率，75%的男性血迹和50%的女性血迹能够明确鉴定。
