The determination of the sex of an individual from a dried bloodstain using radioimmunoassay of testosterone, progesterone, and estradiol-17 beta.

A radioimmunoassay (RIA) technique was developed for the purpose of determining the sex of an individual by measuring the steroids testosterone (T), progesterone (P), and estradiol-17 beta (E2) in dried bloodstains. The steroid values from a single bloodstain are reported as ratios T/P, T/E2, and P/E2. The results reported include data on the validation of this technique, results of analysis of 146 duplicate bloodstains representing 112 samples from females and 34 samples from males as controls, and results of analysis of 114 duplicate bloodstains representing 60 from females and 54 from males that were submitted as a blind study to test the accuracy of this RIA technique in determining the sex of an individual from a dried bloodstain.