Miyata S, Nakamura S, Nagata H, Kameyama M
J Neurol Sci. 1983 Oct;61(2):283-93. doi: 10.1016/0022-510x(83)90012-6.
The manganese distribution in the cross-section of the cervical, thoracic and lumbar portion of the spinal cords from 7 autopsied cases with amyotrophic lateral sclerosis (ALS) and 6 control subjects were determined by radiochemical neutron activation analysis. It was possible to determine 1 ng levels of manganese content accurately in the small tissues about 1 mg of dried weight using wet ashing and chemical separation after neutron activation. The dried weight of spinal cord was about 1/3 of the wet weight. Manganese concentration in the wet tissue was calculated from the ratio of dried to wet weight. In the anterior horn of the cervical cords of ALS, manganese concentration was the highest; 1.75 +/- 0.39 ng/mg of dried weight and 0.59 +/- 0.09 ng/mg of wet weight, respectively. These were significantly higher (P less than 0.01) compared to those in controls; 1.02 +/- 0.12 ng/mg of dried weight and 0.35 +/- 0.04 ng/mg of wet weight, respectively. The elevation of manganese level in the spinal cords of ALS was more prominent in the anterior horn and lateral column than in the posterior column both in dried and wet tissues. Since manganese inhibits neuronal transmission, it is likely that neurological degenerative changes occur as a result of local disturbances of manganese metabolism in the spinal cord of ALS.
通过放射化学中子活化分析，测定了7例肌萎缩侧索硬化症（ALS）尸检病例和6例对照者脊髓颈段、胸段和腰段横截面中的锰分布。在中子活化后，采用湿灰化和化学分离方法，能够准确测定约1mg干重的小组织中1ng水平的锰含量。脊髓干重约为湿重的1/3。根据干重与湿重的比例计算湿组织中的锰浓度。在ALS患者颈髓前角，锰浓度最高，干重分别为1.75±0.39ng/mg，湿重为0.59±0.09ng/mg。与对照组相比，这些值显著更高（P<0.01），对照组干重分别为1.02±0.12ng/mg，湿重为0.35±0.04ng/mg。在干组织和湿组织中，ALS患者脊髓中锰水平的升高在前角和侧柱比后柱更明显。由于锰会抑制神经传递，ALS患者脊髓中锰代谢的局部紊乱可能导致神经退行性变化。
