Darroudi F, Natarajan A T
Mutat Res. 1983 Nov;124(2):179-89. doi: 10.1016/0165-1218(83)90178-7.
Heparinized whole-blood samples from 4 normal donors were treated with resorcinol 24 h after initiation of cultures. Then, after being cultured for 24 and 48 h, the lymphocytes were fixed and the frequencies of chromosomal aberrations and sister-chromatid exchanges (SCEs) were determined. The results from two different experiments indicate that, even at a low concentration of 0.002%, resorcinol was capable of increasing the frequency of chromosomal aberrations. Lymphocytes fixed at 48 h after the start of culture had a lower frequency of aberrations induced by resorcinol than those fixed at 72 h. In the SCE test in human lymphocytes, resorcinol was negative. Parallel experiments with Chinese hamster ovary (CHO) cells indicate that resorcinol is not effective in inducing either chromosomal aberrations or SCEs in these cells. The induced chromosomal aberrations were analysed as induced micronuclei in bone marrow of mice; and in a test in vitro with human diploid fibroblasts, resorcinol was also negative.
来自4名正常供体的肝素化全血样本在培养开始24小时后用间苯二酚处理。然后，在培养24小时和48小时后，固定淋巴细胞并测定染色体畸变和姐妹染色单体交换（SCE）的频率。两个不同实验的结果表明，即使在低浓度0.002%时，间苯二酚也能够增加染色体畸变的频率。培养开始后48小时固定的淋巴细胞，间苯二酚诱导的畸变频率低于72小时固定的淋巴细胞。在人类淋巴细胞的SCE试验中，间苯二酚呈阴性。用中国仓鼠卵巢（CHO）细胞进行的平行实验表明，间苯二酚对诱导这些细胞的染色体畸变或SCE均无效。将诱导的染色体畸变分析为小鼠骨髓中的诱导微核；在用人二倍体成纤维细胞进行的体外试验中，间苯二酚也呈阴性。
