Determination of serum concentrations of some aminoglycoside antibiotics by use of the fluorescent immunoassay method.

In serum samples of 20 children the concentration of amikacin, gentamicin and tobramycin was determined paralelly by use of conventional microbiological method and fluoroimmunoassay technique. Peak concentration of tested aminoglycosides was observed 60 min after the first administration of the drug. The "trough level" of amikacin was lower than 10 micrograms/ml and that of gentamicin and tobramycin lower than 2 microgram/ml. Serum concentration of aminoglycosides in samples tested by microbiological assays and fluoroimmunoassay test was similar, correlation coefficient was 0.93.