心肌细胞中激素刺激的脂解作用。
Hormone-stimulated lipolysis in cardiac myocytes.
作者信息
Palmer W K, Kane T A
出版信息
Biochem J. 1983 Oct 15;216(1):241-3. doi: 10.1042/bj2160241.
Abstract
Type L hormone-sensitive lipase (HSL) activity was increased approx. 35% above control in cardiac myocytes incubated for 15 min with 5 nM-adrenaline. Concomitantly. adrenaline-stimulated myocytes had a lower triacylglycerol content, released more non-esterified fatty acid and had a higher intracellular concentration of cyclic AMP than did myocytes incubated without hormone. The lipase activity measured in adrenaline-stimulated and non-stimulated myocytes was stable in acetone/diethyl ether, stimulated by serum and inhibited by NaCl. These properties are consistent with the type L designation of this HSL. The finding that type L HSL is stimulated by adrenaline indicates that the enzyme that is being activated is found in the cell and not associated with an extracellular compartment of the myocardium.
摘要
L型激素敏感性脂肪酶（HSL）活性在用5 nM肾上腺素孵育15分钟的心肌细胞中比对照增加了约35%。同时，肾上腺素刺激的心肌细胞的三酰甘油含量较低，释放出更多的非酯化脂肪酸，并且细胞内的环磷酸腺苷浓度比未用激素孵育的心肌细胞更高。在肾上腺素刺激和未刺激的心肌细胞中测得的脂肪酶活性在丙酮/乙醚中稳定，受血清刺激并被氯化钠抑制。这些特性与该HSL的L型命名一致。L型HSL受肾上腺素刺激这一发现表明，被激活的酶存在于细胞中，而不是与心肌的细胞外区室相关。
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