Hayes J D, Gilligan D, Chapman B J, Beckett G J
Clin Chim Acta. 1983 Oct 31;134(1-2):107-21. doi: 10.1016/0009-8981(83)90189-4.
A purification scheme is described for six human hepatic glutathione S-transferases from a single liver. Five of the transferases comprised Ya monomers and had a molecular mass of 44 000. The remaining enzyme comprised Yb monomers and had a molecular mass of 47 000. Data are presented demonstrating that there are at least two distinct Ya monomers. A radioimmunoassay has been developed that has sufficient precision and sensitivity to allow direct measurement of glutathione S-transferase concentrations in unextracted plasma. A comparison of aminotransferase and glutathione S-transferase levels, in three patients who had taken a paracetamol overdose, indicated that glutathione S-transferase measurements provided a far more sensitive index of hepatocellular integrity than the more conventional aminotransferase measurements.
本文描述了一种从单个肝脏中纯化六种人肝谷胱甘肽S-转移酶的方案。其中五种转移酶由Ya单体组成，分子量为44000。其余的酶由Yb单体组成，分子量为47000。所提供的数据表明至少存在两种不同的Ya单体。已开发出一种放射免疫测定法，其具有足够的精密度和灵敏度，可直接测量未提取血浆中的谷胱甘肽S-转移酶浓度。对三名服用过量扑热息痛患者的转氨酶和谷胱甘肽S-转移酶水平进行比较，结果表明，与更传统的转氨酶测量相比，谷胱甘肽S-转移酶测量能提供更敏感的肝细胞完整性指标。
