Moreland F M, Sheu C J
Drug Chem Toxicol. 1983;6(6):537-48. doi: 10.3109/01480548309017808.
A comparison of the fertility and cytogenetic analyses and the combination of both techniques as generally used in the heritable translocation assay was conducted with triethylenemelamine (TEM). CD-1 mice were used as the F0 generation and were divided into two groups that received i.p. either distilled water (control) or TEM at 0.15-0.20 mg/kg. A total of 226 F1 males were generated (125 control, 101 TEM group) and were subjected to both sequential fertility assessment and direct cytogenetic analysis. A litter size of 10 was used for the fertility assessment method and 25 cells per animal were scored for the direct cytogenetic analysis; 20 animals were initially identified as translocation carriers based on the fertility analysis and 16 animals were identified by cytogenetic analysis. When these data were compared and the discrepancies resolved, each method separately identified 16 translocation carriers (15 animals common to both methods). With the combination of data for both techniques, as is done in the heritable translocation assay, a total of 17 translocation heterozygotes were identified. Use of either the fertility method alone or the cytogenetic method as routinely used would have resulted in the separate identification of 16 translocation carriers per procedure, with each method failing to identify one carrier.
使用三亚乙基三聚氰胺（TEM）对遗传性易位试验中通常使用的生育力和细胞遗传学分析以及两种技术的组合进行了比较。将CD-1小鼠用作F0代，并分为两组，分别腹腔注射蒸馏水（对照组）或0.15 - 0.20 mg/kg的TEM。共产生了226只F1雄性小鼠（125只对照组，101只TEM组），并对其进行了连续生育力评估和直接细胞遗传学分析。生育力评估方法采用每窝产仔数为10只，直接细胞遗传学分析每只动物计数25个细胞；最初根据生育力分析确定20只动物为易位携带者，通过细胞遗传学分析确定16只动物为易位携带者。当比较这些数据并解决差异时，每种方法分别鉴定出16只易位携带者（两种方法共有15只动物）。按照遗传性易位试验的做法，将两种技术的数据结合起来，共鉴定出17只易位杂合子。单独使用生育力方法或常规使用的细胞遗传学方法，每个程序将分别鉴定出16只易位携带者，每种方法都未能鉴定出一只携带者。
