Sheu C W, Roginski H B, Blaschka A M, Laverson S, Ortel C D
Mutat Res. 1982 Aug;97(4):257-65. doi: 10.1016/0165-1161(82)90025-5.
A study was conducted to determine the extent to which translocation carriers can be missed in the heritable translocation test. CD-1 male mice were treated with the mutagen triethylenemelamine and bred to untreated females and male progeny were subjected to fertility and cytogenetic analyses. Fertility testing involved mating 1 male to 3 virgin females and subjecting the females to uterine analysis. The males were classified as having normal or reduced fertility according to the number of live implants produced by the females. The cytogenetic analysis involved scoring 25 primary spermatocytes per mouse for translocation multivalents. A total of 103 male progeny were analyzed. Evaluation of fertility and cytogenetic data confirmed the presence of 18 translocation heterozygotes among the male progeny. On the basis of fertility testing, 1 translocation heterozygote was classified as normal (false negative) based on the production of 11 or more live implants by at least 1 mated female. On the basis of cytogenetic analysis of 25 cells per mouse, 1 partially sterile male was classified as normal (false negative); however, analysis of additional cells showed that this mouse was a translocation heterozygote. The study demonstrates the importance of evaluating fertility and cytogenetic criteria to minimize the extent of misclassification in conducting a heritable translocation test.
进行了一项研究，以确定在遗传性易位试验中易位携带者被漏检的程度。将CD - 1雄性小鼠用诱变剂三亚乙基三聚氰胺处理，然后与未处理的雌性小鼠交配，对雄性后代进行生育力和细胞遗传学分析。生育力测试包括将1只雄性与3只未交配过的雌性交配，并对雌性进行子宫分析。根据雌性产生的活胚胎植入数，将雄性分为生育力正常或降低。细胞遗传学分析包括对每只小鼠的25个初级精母细胞进行易位多价体评分。总共分析了103只雄性后代。对生育力和细胞遗传学数据的评估证实，雄性后代中有18只易位杂合子。根据生育力测试，基于至少1只交配雌性产生11个或更多活胚胎植入，1只易位杂合子被归类为正常（假阴性）。根据每只小鼠25个细胞的细胞遗传学分析，1只部分不育的雄性被归类为正常（假阴性）；然而，对更多细胞的分析表明，这只小鼠是易位杂合子。该研究证明了评估生育力和细胞遗传学标准对于在进行遗传性易位试验时尽量减少错误分类程度的重要性。
