放射性碳对雄性小鼠生殖细胞的遗传效应。

Genetic effects of radiocarbon in reproductive cells of male mice.

作者信息

Pomerantseva M D, Ramaiya L K, Vilkina G A, Shevchenko V A, Vasilenko I J, Lyaginskaya A M, Istomina A G

出版信息

Mutat Res. 1983 Dec;122(3-4):341-6. doi: 10.1016/0165-7992(83)90017-9.

Abstract

The genetic effect of incorporated radiocarbon was studied after single, long-term (33 days) and chronic (6 and 12 months) treatment of male mice (CBA X C57B1) F1 with [14C]glucose. The genetic effect in male germ cells was estimated by 3 tests: DLM frequency in post- and pre-meiotic cells, RT frequency in stem spermatogonia and frequency of abnormal sperm heads. Absorbed doses in the gonads were: 0.22, 0.50 and 1.01 Gy, after a single exposure; 0.74 and 1.47 Gy, after long-term exposures; and 0.006 and 0.031 Gy, after chronic exposure for 6 months; and 0.013 and 0.066 Gy, for 12 months. The results suggest that DLM frequency in post-meiotic cells increased linearly with increasing the dose of 14C single and long-term exposures at a dose of 1.47 Gy only. A chronic treatment with [14C]glucose induced no increase in DLM frequency. RT frequency in stem spermatogonia was statistically significantly higher than the control level after the single and long-term exposure to 14C. A comparison of the results with the results of external single and chronic gamma-irradiation allows the conclusion that the relative genetic efficiency of radiocarbon as compared with that of gamma-rays is about 1.

摘要

用[¹⁴C]葡萄糖对雄性（CBA×C57B1）F1代小鼠进行单次、长期（33天）和慢性（6个月和12个月）处理后，研究了掺入的放射性碳的遗传效应。通过3项试验评估雄性生殖细胞中的遗传效应：减数分裂后和减数分裂前细胞中的双着丝粒体（DLM）频率、精原干细胞中的染色体易位（RT）频率以及异常精子头部的频率。性腺吸收剂量分别为：单次照射后为0.22、0.50和1.01 Gy；长期照射后为0.74和1.47 Gy；慢性照射6个月后为0.006和0.031 Gy；慢性照射12个月后为0.013和0.066 Gy。结果表明，仅在剂量为1.47 Gy的¹⁴C单次和长期照射下，减数分裂后细胞中的DLM频率随剂量增加呈线性增加。用[¹⁴C]葡萄糖进行慢性处理未导致DLM频率增加。单次和长期暴露于¹⁴C后，精原干细胞中的RT频率在统计学上显著高于对照水平。将结果与外部单次和慢性γ射线照射的结果进行比较可以得出结论，与γ射线相比，放射性碳的相对遗传效率约为1。