Keimig S D, Kirby K W, Morgan D P, Keiser J E, Hubert T D
Xenobiotica. 1983 Jul;13(7):415-20. doi: 10.3109/00498258309052279.
1-Hydroxypyrene is a major metabolite of pyrene given orally to pigs. A method for isolating the metabolite from urine is described, utilizing C18-adsorbent cartridges, h.p.l.c. separation, and fluorescence detection. Unconjugated 1-hydroxypyrene can be detected in the urine of pigs following oral administration of as little as 1 mg pyrene. Identity of the 1-hydroxypyrene was confirmed by u.v.-absorption and fluorescence spectrometry, mass spectrometry, and by comparison with the retention time characteristic of synthetic 1-hydroxypyrene.
1-羟基芘是猪口服芘后的主要代谢产物。本文描述了一种从尿液中分离该代谢产物的方法，该方法利用C18吸附柱、高效液相色谱分离和荧光检测。口服低至1毫克芘后，猪尿液中即可检测到未结合的1-羟基芘。通过紫外吸收和荧光光谱法、质谱法以及与合成1-羟基芘的保留时间特征进行比较，确认了1-羟基芘的身份。
