Bondarenko N D
Arkh Anat Gistol Embriol. 1983 Nov;85(11):66-72.
In 20 dogs with a corrugated stenosis of the pulmonary trunk, the state of the intramural neural system has been studied by means of some histological techniques. For correction a by-pass cardio-pulmonary shunt has been used. Fifteen dogs have been used as controls, in 5 of them stenosis of the pulmonary trunk without correction has been induced, in 5--the right ventricular wall has been dissected and the heart wound has been saturated ; in 5--thoracotomy has been performed. Various parts of the cardiac wall have been investigated during the interval 2 hours--360 days. Changes in the cardiac intramural apparatuses at the time up to the 7th day can be considered as nonspecific in response to the surgical trauma. They are similarly manifested in all parts of the cardiac wall in the animals of all the groups studied. Subsequently, destructive changes of neurocytes are the most pronounced. The cells decrease in their size, the nuclei are not detected, RNA content is sharply decreased. When stained after Nissl, "ghost-cells" are seen. Thick neural fibers undergo fragmentation and granular decay. The receptors are subjected to the most sharp changes. These processes are most pronounced up to the 30th day. Further (when stenosis of the pulmonary trunk is corrected with the conduit), restorative processes prevail. In the bodies of neurocytes pironinophilia increases, RNA content rises, young receptors with soft weakly argerophile fibrillae are often detected. Thus, up to 4-6 months the state of the intramural neural apparatuses approaches the normal state. The results of the investigation performed make it possible to conclude that the correction method for the pulmonary trunk stenosis is effective. Owing to this method it is possible to avoid destructive changes that progressively develop in animals with a noncorregated stenosis of the pulmonary trunk.
在20只患有肺动脉波纹状狭窄的犬中，通过一些组织学技术研究了壁内神经系统的状态。为进行矫正，采用了心肺分流旁路术。15只犬用作对照，其中5只诱发了未矫正的肺动脉狭窄，5只切开右心室壁并使心脏创口浸润，5只进行了开胸手术。在2小时至360天的时间段内对心脏壁的各个部分进行了研究。在第7天之前，心脏壁内结构的变化可被视为对手术创伤的非特异性反应。在所有研究组的动物中，心脏壁的所有部分都有类似表现。随后，神经细胞的破坏性变化最为明显。细胞体积减小，细胞核消失，RNA含量急剧下降。经尼氏染色后可见“幽灵细胞”。粗大的神经纤维发生断裂和颗粒状退变。感受器变化最为剧烈。这些过程在第30天之前最为明显。进一步（当用导管矫正肺动脉狭窄时），修复过程占主导。神经细胞体内嗜派洛宁性增加，RNA含量上升，常检测到带有柔软、弱嗜银纤维的年轻感受器。因此，在4至6个月内，壁内神经结构的状态接近正常状态。所进行的研究结果可以得出结论，肺动脉狭窄的矫正方法是有效的。由于这种方法，可以避免在患有非波纹状肺动脉狭窄的动物中逐渐发展的破坏性变化。
