烟酰胺腺嘌呤二核苷酸（NAD⁺）在完整分离的植物线粒体与悬浮介质之间的缓慢被动扩散。
Slow passive diffusion of NAD+ between intact isolated plant mitochondria and suspending medium.
作者信息
Neuburger M, Douce R
出版信息
Biochem J. 1983 Nov 15;216(2):443-50. doi: 10.1042/bj2160443.
Abstract
Isolated potato (Solanum tuberosum) tuber mitochondria purified by isopycnic centrifugation in density gradients of Percoll were found to be highly intact, to be devoid of extramitochondrial contaminations and to retain a high rate of O2 consumption. When suspended in a medium that avoided rupture of the outer membrane, intact purified mitochondria progressively lost their NAD+ content by passive diffusion. This led to a slow decrease of oxoglutarate-dependent O2 consumption by isolated mitochondria. Addition of NAD+ to the medium restored the initial State-3 rate of oxoglutarate oxidation. The rate of NAD+ accumulation in the matrix space was concentration-dependent, exhibited Michaelis-Menten kinetics and was strongly inhibited by the analogue N-4-azido-2-nitrophenyl-4-aminobutyryl-NAD+.
摘要
通过在Percoll密度梯度中进行等密度离心纯化得到的离体马铃薯（茄属）块茎线粒体被发现高度完整，没有线粒体外的污染物，并保持较高的氧气消耗率。当悬浮在避免外膜破裂的培养基中时，完整的纯化线粒体通过被动扩散逐渐失去其NAD⁺含量。这导致离体线粒体依赖于酮戊二酸的氧气消耗缓慢下降。向培养基中添加NAD⁺可恢复酮戊二酸氧化的初始状态3速率。NAD⁺在基质空间中的积累速率是浓度依赖性的，表现出米氏动力学，并受到类似物N-4-叠氮基-2-硝基苯基-4-氨基丁酰-NAD⁺的强烈抑制。
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