动脉平滑肌细胞中液相胞饮作用对温度、细胞ATP浓度和细胞骨架系统的依赖性。
Dependence of fluid-phase pinocytosis in arterial smooth-muscle cells on temperature, cellular ATP concentration and the cytoskeletal system.
作者信息
Muir E M, Bowyer D E
出版信息
Biochem J. 1983 Nov 15;216(2):467-73. doi: 10.1042/bj2160467.
125I-labelled poly(vinylpyrrolidone) was used as a marker of fluid-phase pinocytosis in cultured pig arterial smooth-muscle cells. The rate of pinocytosis was temperature-dependent. A decrease in cellular ATP concentrations as a result of inhibition of either glycolysis or oxidative phosphorylation was associated with a similar decrease in pinocytosis. A microfibrillar-disruptive agent, cytochalasin B, caused a concentration-dependent stimulation of pinocytosis, whereas the microtubular-disruptive agents colchicine and vinblastine decreased pinocytosis to approximately half of control values at all concentrations used. These results indicate that fluid-phase pinocytosis in smooth-muscle cells is dependent on a continuing supply of energy and the integrity of the microtubules. Furthermore, microfilaments appear to exert a certain degree of constraint on pinocytosis, possibly by restricting invagination of the plasma membrane.
125I标记的聚（乙烯基吡咯烷酮）被用作培养的猪动脉平滑肌细胞中液相胞饮作用的标志物。胞饮速率取决于温度。由于糖酵解或氧化磷酸化受到抑制而导致的细胞ATP浓度降低，与胞饮作用的类似降低相关。一种微原纤维破坏剂，细胞松弛素B，引起胞饮作用的浓度依赖性刺激，而微管破坏剂秋水仙碱和长春花碱在所有使用的浓度下都将胞饮作用降低到对照值的大约一半。这些结果表明，平滑肌细胞中的液相胞饮作用依赖于能量的持续供应和微管的完整性。此外，微丝似乎对胞饮作用施加了一定程度的限制，可能是通过限制质膜的内陷来实现的。
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