Hökfelt T, Smith C B, Peters A, Norell G, Crane A, Brownstein M, Sokoloff L
Brain Res. 1983 Dec 19;289(1-2):311-6. doi: 10.1016/0006-8993(83)90032-x.
It was attempted to improve the resolution of the 2-deoxyglucose method. Two principle changes in the procedure were introduced: the gluing of the sections on to the object slide at--20 degrees C and the application of the emulsion with the loop technique. With this approach autoradiographs with grain accumulations over single cell bodies could be observed in many brain regions in addition to a diffuse activity over neuropil.
人们试图改进2-脱氧葡萄糖法的分辨率。该程序引入了两个主要变化：在-20℃下将切片粘贴到载玻片上，以及采用环技术涂抹乳剂。通过这种方法，除了神经毡上的弥漫性活动外，在许多脑区还能观察到单细胞体上有颗粒聚集的放射自显影片。
