Glass G A, Gershon H, Gershon D
Exp Hematol. 1983 Nov;11(10):987-95.
Erythrocytes from young and old WF rats were separated by discontinuous density gradient centrifugation. The density and mean life span of the erythrocytes were correlated with cell age by 59Fe pulse labeling. A mean half-life of 40 and 25 days was measured for erythrocytes in young and old animals, respectively, with a corresponding 4-fold increase of young cells and a 2.3-fold increase in circulating reticulocytes in old animals. Thus, the old rat has a chronologically younger population of circulating erythrocytes than the young animal. The mean hemoglobin concentration of red cells from old, but not young animals, was found to decrease from 18.5 +/- 2.1 pg to 11.8 +/- 1.8 pg per cell with increasing cell age. Premature exposure of a surface antigen, which is recognized by antibodies present in the serum, was observed in cells of old animals. This antigen-antibody complex is presumed to trigger sequestration by phagocytosis of senescent erythrocytes. The contribution of the observed age-related changes to early erythrocyte sequestration in old animals is discussed.
通过不连续密度梯度离心法分离年轻和老年WF大鼠的红细胞。通过59Fe脉冲标记，将红细胞的密度和平均寿命与细胞年龄相关联。年轻和老年动物红细胞的平均半衰期分别测定为40天和25天，老年动物中年轻细胞相应增加4倍，循环网织红细胞增加2.3倍。因此，老年大鼠循环红细胞群体的年龄在时间顺序上比年轻动物的更年轻。发现老年动物而非年轻动物的红细胞平均血红蛋白浓度随细胞年龄增加从每细胞18.5±2.1皮克降至11.8±1.8皮克。在老年动物的细胞中观察到一种表面抗原过早暴露，该抗原可被血清中存在的抗体识别。这种抗原-抗体复合物被认为会触发衰老红细胞通过吞噬作用被隔离。讨论了观察到的与年龄相关的变化对老年动物早期红细胞隔离的作用。
