O'Brodovich H, Andrew M, Silver R, Coates G
J Appl Physiol Respir Environ Exerc Physiol. 1983 Dec;55(6):1743-7. doi: 10.1152/jappl.1983.55.6.1743.
Experiments were performed to determine whether activation of the coagulation cascade was required for pulmonary vascular permeability to increase during microembolization of the lung. For 30-45 min air microemboli were intravenously infused (0.05-0.10 ml X kg-1 X min-1) into awake sheep with chronic lung-lymph fistulas and anesthetized mongrel dogs. During embolization the pulmonary arterial pressure increased, and O2 partial pressure (PaO2) fell by more than 20 Torr (P less than 0.01). Subsequently lymph flow nearly tripled without a change in the lymph-to-plasma protein concentration ratio. Partial thromboplastin and prothrombin times, biological activity of antithrombin III, and circulating concentration of 125I-labeled dog or sheep fibrinogen did not change during or following air infusion. In two additional sheep an intravenous infusion of thrombin at 0.6 U X kg-1 X min-1 for 15 min resulted in a 20% decrease in 125I-labeled sheep fibrinogen concentration without a change in pulmonary arterial pressure or PaO2. We conclude that air microembolization can increase permeability to water and protein without a detectable activation of the coagulation cascade in the sheep or dog.
进行实验以确定在肺部微栓塞过程中，肺血管通透性增加是否需要凝血级联反应的激活。将空气微栓子以静脉注射的方式（0.05 - 0.10 ml·kg⁻¹·min⁻¹）持续输注30 - 45分钟，对象为患有慢性肺淋巴瘘的清醒绵羊和麻醉的杂种狗。在栓塞过程中，肺动脉压升高，氧分压（PaO₂）下降超过20 Torr（P < 0.01）。随后淋巴流量几乎增加了两倍，而淋巴与血浆蛋白浓度比没有变化。部分凝血活酶时间和凝血酶原时间、抗凝血酶III的生物活性以及¹²⁵I标记的狗或绵羊纤维蛋白原的循环浓度在空气输注期间或之后均未改变。在另外两只绵羊中，以0.6 U·kg⁻¹·min⁻¹的速度静脉输注凝血酶15分钟，导致¹²⁵I标记的绵羊纤维蛋白原浓度降低20%，而肺动脉压或PaO₂没有变化。我们得出结论，空气微栓塞可增加水和蛋白质的通透性，而在绵羊或狗中未检测到凝血级联反应的激活。
