Kono M, Sasatsu M, Ubukata K, Konno M, Fujii R
Antimicrob Agents Chemother. 1978 Apr;13(4):691-4. doi: 10.1128/AAC.13.4.691.
Multiply drug-resistant strains of Staphylococcus aureus were isolated from pediatric patients with severe staphylococcal infections in 1974 through 1976. Resistance to benzylpenicillin, erythromycin, and kanamycin was jointly eliminated without exception from these multiply drug-resistant strains by treatment with ethidium bromide. It was also found that the triple drug resistance in a representative strain, TK512-200, was always transduced to a susceptible strain simultaneously. Moreover, a single class of plasmid deoxyribonucleic acid was isolated from a transductant and found to be 14.4 +/- 0.6 mum in length, with a molecular weight corresponding to 29.8 x 10(6). From these results, it is concluded that the plasmid (pTU512) is a new one, mediating resistance to penicillin, erythromycin, and kanamycin.
1974年至1976年期间，从患有严重葡萄球菌感染的儿科患者中分离出多重耐药性金黄色葡萄球菌菌株。用溴化乙锭处理这些多重耐药菌株后，对苄青霉素、红霉素和卡那霉素的耐药性无一例外地同时消除。还发现，代表性菌株TK512 - 200中的三重耐药性总是同时转导到一个敏感菌株中。此外，从一个转导子中分离出一类单一的质粒脱氧核糖核酸，发现其长度为14.4 +/- 0.6微米，分子量相当于29.8 x 10(6)。根据这些结果，可以得出结论，该质粒（pTU512）是一种新的质粒，介导对青霉素、红霉素和卡那霉素的耐药性。
