Emslie K R, Ozanne N R, Nade S M
J Pathol. 1983 Oct;141(2):157-67. doi: 10.1002/path.1711410206.
A simple and reproducible model of acute haematogenous staphylococcal osteomyelitis is described. Twenty-nine day-old chickens were inoculated intravenously with 10(4)-10(8) viable organisms Staphylococcus aureus per kg body weight and were killed 1-8 days after inoculation. Macroscopic septic foci could be detected within 24 hr of inoculation and were most commonly situated in the metaphyseal region of the proximal tibia and distal femur. Lesions in other organs were not observed. The production of osteomyelitis was dependent on the bacterial inoculum size. It was estimated that 5.5 X 10(5) viable organisms per kg body weight of chicken were required to produce osteomyelitis in 50 per cent of injected chickens. Chicken weights were monitored throughout the experiment. A close negative correlation existed between the logarithm of the bacterial inoculum size and the chicken growth rate in the first 24 hr following inoculation (r = -0.968, P less than 0.01). The chicken growth rate was therefore used as an accurate predictor of osteomyelitis in individual chickens.
本文描述了一种简单且可重复的急性血源性金黄色葡萄球菌骨髓炎模型。将29日龄的鸡按每千克体重静脉接种10⁴ - 10⁸个活的金黄色葡萄球菌，接种后1 - 8天处死。接种后24小时内可检测到肉眼可见的化脓病灶，最常见于胫骨近端和股骨远端的干骺端区域。未观察到其他器官有病变。骨髓炎的发生取决于细菌接种量。据估计，每千克体重的鸡接种5.5×10⁵个活的金黄色葡萄球菌时，50%的接种鸡会发生骨髓炎。在整个实验过程中监测鸡的体重。接种后最初24小时内，细菌接种量的对数与鸡的生长速率之间存在密切的负相关（r = -0.968，P < 0.01）。因此，鸡的生长速率被用作个体鸡发生骨髓炎的准确预测指标。
