Hashizume S, Nagao S, Tanaka N, Sekiguchi F, Kohno M, Ogawa H, Yoshino Y, Seki K, Ishihara K
Jpn J Exp Med. 1983 Apr;53(2):77-85.
The antitumor effects of human fibroblast interferon (HuIFN-beta) on three lines of human malignant melanoma (AM-1, SK-14, SK-2) were studied using a nude mouse-human tumor xenograft system. The sensitivity of melanoma to interferon in relation to melanin productivity was investigated. Intratumoral administration of 6 X 10(5) IU of HuIFN-beta significantly inhibited proliferation of AM-1 and SK-14, but did not inhibit that of SK-2. The sensitivity to HuIFN-beta was in the order of SK-14, AM-1 and SK-2, and was well correlated with the susceptibility of cell division observed histologically. Although these tumors differ in production of melanin, difference in sensitivity to HuIFN-beta due to melanin productivity was not clear. The relationship between the antitumor effect and the administration method was studied with SK-14, which was the most sensitive to HuIFN-beta. Antitumoral activities depending on the routes of administration were in the order of intratumoral, peritumoral-subcutaneous and intraperitoneal. Intratumoral and subcutaneous administrations of high doses brought about complete disappearance of the tumor cells or decrease in size of the tumor mass.
利用裸鼠-人肿瘤异种移植系统研究了人成纤维细胞干扰素（HuIFN-β）对三株人恶性黑色素瘤细胞系（AM-1、SK-14、SK-2）的抗肿瘤作用。研究了黑色素瘤对干扰素的敏感性与黑色素生成能力之间的关系。瘤内注射6×10⁵国际单位的HuIFN-β可显著抑制AM-1和SK-14的增殖，但对SK-2无抑制作用。对HuIFN-β的敏感性顺序为SK-14、AM-1和SK-2，且与组织学观察到的细胞分裂敏感性密切相关。尽管这些肿瘤在黑色素生成方面存在差异，但黑色素生成能力导致的对HuIFN-β敏感性差异并不明显。以对HuIFN-β最敏感的SK-14研究了抗肿瘤作用与给药方式之间的关系。不同给药途径的抗肿瘤活性顺序为瘤内、瘤周皮下和腹腔内。高剂量的瘤内和皮下给药可使肿瘤细胞完全消失或肿瘤块体积减小。
