钙依赖性高尔基体囊泡融合及组织蛋白酶B在大鼠肝脏中前清蛋白转化为清蛋白过程中的作用
Calcium-dependent Golgi-vesicle fusion and cathepsin B in the conversion of proalbumin into albumin in rat liver.
作者信息
Quinn P S, Judah J D
出版信息
Biochem J. 1978 May 15;172(2):301-9. doi: 10.1042/bj1720301.
Abstract
- An enzyme from rat liver that converts proalbumin into albumin is described. Partial purification, inhibitor studies and the conditions for maximum activity suggest that the enzyme is cathepsin B. 2. A membrane-bound enzyme, located mainly in lysosomes, also converts proalbumin into albumin. This appears to be a membrane-bound form of cathepsin B. 3. Isolated Golgi vesicles, incubated under conditions suitable for cathepsin B, convert endogenous proalbumin into albumin. 4. This conversion in Golgi vesicles has an absolute requirement for Ca2+ at micromolar concentrations. Mg2+ does not affect or substitute for Ca2+. Both the proalbumin and the albumin formed from it are intravesicular. 5. Converting activity is enhanced by pretreatment with the known chemical fusogen, poly(ethyleneglycol). 6. Vesicles preincubated at pH above 7 in the presence of dithiothreitol show a marked fall in converting activity. This can be partially restored by incubation with native vesicles. These results suggest that vesicle fusion is a requirement for conversion of proalbumin into albumin.
摘要
- 本文描述了一种来自大鼠肝脏的可将前白蛋白转化为白蛋白的酶。部分纯化、抑制剂研究以及最大活性条件表明该酶是组织蛋白酶B。2. 一种主要位于溶酶体中的膜结合酶也可将前白蛋白转化为白蛋白。这似乎是组织蛋白酶B的膜结合形式。3. 在适合组织蛋白酶B的条件下孵育分离出的高尔基体囊泡，可将内源性前白蛋白转化为白蛋白。4. 高尔基体囊泡中的这种转化绝对需要微摩尔浓度的Ca2+。Mg2+不影响也不能替代Ca2+。前白蛋白及其形成的白蛋白均在囊泡内。5. 用已知的化学融合剂聚乙二醇预处理可增强转化活性。6. 在二硫苏糖醇存在下于pH高于7的条件下预孵育的囊泡，其转化活性显著下降。与天然囊泡一起孵育可部分恢复该活性。这些结果表明囊泡融合是前白蛋白转化为白蛋白的必要条件。
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