一种从低熔点琼脂糖中分离后连接DNA的方法。
A method for the ligation of DNA following isolation from low melting temperature agarose.
作者信息
Burns D M, Beacham I R
出版信息
Anal Biochem. 1983 Nov;135(1):48-51. doi: 10.1016/0003-2697(83)90728-5.
PMID:6670747
Abstract
A rapid, simple, and reliable method is presented for the isolation and subsequent ligation of DNA from agarose gels. The technique involves the use of low melting temperature agarose, but with the inclusion of bovine serum albumin or gelatin to the ligation reaction.
摘要
本文介绍了一种从琼脂糖凝胶中分离并随后连接DNA的快速、简单且可靠的方法。该技术使用低熔点琼脂糖，但在连接反应中加入牛血清白蛋白或明胶。
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