Greilhuber J, Volleth M, Loidl J
Can J Genet Cytol. 1983 Dec;25(6):554-60. doi: 10.1139/g83-084.
A direct Feulgen-cytophotometric comparison of the genomic DNA content (C value) was performed between the liliaceous plant species Allium cepa and a number of animal species to reassess the genome size ratios between plants and animals. These appeared unduly ambiguous as a consequence of divergent picogram estimates in several animal reference species. Taking 1C = 16.75 pg for Allium cepa, the estimates were (1C value in picograms): man, 3.11; Indian muntjak CCL 157 cell line, 2.68; domestic pig, 2.79; Chinese hamster, 2.66; CHO cell line, 2.73; laboratory rat, 2.65; mouse, 3.04; rat kangaroo Pt-K2 cell line, 4.21; fowl, 1.16; and the green toad, 4.30. These values are consistent with a number of independent absolute and relative DNA content determinations reported for animals, and therefore define a coherent set of animal and plant reference values for genome size determinations.
对葱属植物洋葱和一些动物物种的基因组DNA含量(C值)进行了直接的福尔根细胞光度测定比较,以重新评估植物和动物之间的基因组大小比率。由于几种动物参考物种的皮克估计值存在差异,这些比率显得异常模糊。以洋葱的1C = 16.75皮克为基准,估计值如下(皮克为单位的1C值):人类,3.11;印度麂CCL 157细胞系,2.68;家猪,2.79;中国仓鼠,2.66;CHO细胞系,2.73;实验大鼠,2.65;小鼠,3.04;大鼠袋鼠Pt-K2细胞系,4.21;家禽,1.16;绿蟾蜍,4.30。这些值与报道的许多关于动物的独立绝对和相对DNA含量测定结果一致,因此为基因组大小测定定义了一组连贯的动植物参考值。