Sohn L L, Saleh O A, Facer G R, Beavis A J, Allan R S, Notterman D A
Departments of Physics and Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):10687-90. doi: 10.1073/pnas.200361297.
Measuring the DNA content of eukaryotic cells is a fundamental task in biology and medicine. We have observed a linear relationship between the DNA content of eukaryotic cells and the change in capacitance that is evoked by the passage of individual cells across a 1-kHz electric field. This relationship is species-independent; consequently, we have developed a microfluidic technique-"capacitance cytometry"-that can be used to quantify the DNA content of single eukaryotic cells and to analyze the cell-cycle kinetics of populations of cells. Comparisons with standard flow cytometry demonstrate the sensitivity of this new technique.
测量真核细胞的DNA含量是生物学和医学中的一项基本任务。我们观察到真核细胞的DNA含量与单个细胞通过1 kHz电场时所引起的电容变化之间存在线性关系。这种关系与物种无关；因此，我们开发了一种微流控技术——“电容细胞术”，可用于量化单个真核细胞的DNA含量，并分析细胞群体的细胞周期动力学。与标准流式细胞术的比较证明了这项新技术的灵敏度。
