透明质酸的构象转变：羧基参与及热效应

Conformational transition of hyaluronic acid: carboxylic group participation and thermal effect.

作者信息

Park J W, Chakrabarti B

出版信息

Biochim Biophys Acta. 1978 Jun 15;541(2):263-9. doi: 10.1016/0304-4165(78)90399-9.

Abstract

The chiroptical, viscosity and titration studies of hyaluronic acid in mixed organic/water solvent show a reversible conformational transition of the molecule depending upon pH, solvent composition, temperature, and molecular weight. Neither methylhyaluronate nor chondroitin undergoes conformational transition of this type. These results indicate that hydrogen bonding between the protonated carboxylic group and carbonyl oxygen of the acetamido group is directly involved in the conformational change. Results with chondroitin provide further support for the 4-fold helical structure that we have proposed for hyaluronic acid in mixed organic/water solvent. The thermal stability of the conformation has been studied under various pH values and solvent compositions.

摘要

在有机/水混合溶剂中对透明质酸进行的旋光、粘度和滴定研究表明，该分子会根据pH值、溶剂组成、温度和分子量发生可逆的构象转变。甲基透明质酸和软骨素均未发生此类构象转变。这些结果表明，质子化羧基与乙酰氨基的羰基氧之间的氢键直接参与了构象变化。软骨素的研究结果进一步支持了我们提出的透明质酸在有机/水混合溶剂中的四重螺旋结构。已在各种pH值和溶剂组成条件下研究了该构象的热稳定性。