用于检测乳糜泻的麦醇溶蛋白抗体评估

Evaluation of gliadin antibodies for detection of coeliac disease.

作者信息

Kilander A F, Dotevall G, Fällström S P, Gillberg R E, Nilsson L A, Tarkowski A

出版信息

Scand J Gastroenterol. 1983 May;18(3):377-83. doi: 10.3109/00365528309181610.

Abstract

To evaluate assay of gliadin antibodies of different immunoglobulin classes as a test for detection of coeliac disease, we analysed sera from 36 adult patients and 8 children with coeliac disease, 62 patients with other gastrointestinal diseases, and 124 blood donors with diffusion-in-gel enzyme-linked immunosorbent assay (DIG-ELISA). Depending on the choice of reference levels for gliadin antibodies of the IgA and IgG classes, respectively, we found a diagnostic sensitivity for coeliac disease of 93-86% and a diagnostic specificity of 95-100%. Determination of gliadin antibodies by DIG-ELISA can thus be used as a test for detection of coeliac disease and selection of patients for small-intestinal biopsy.

摘要

为评估不同免疫球蛋白类别的麦醇溶蛋白抗体检测法作为乳糜泻检测试验的效果，我们采用凝胶扩散酶联免疫吸附测定法（DIG-ELISA）分析了36例成年乳糜泻患者、8例儿童乳糜泻患者、62例患有其他胃肠道疾病的患者以及124名献血者的血清。根据分别针对IgA和IgG类麦醇溶蛋白抗体所选择的参考水平，我们发现乳糜泻的诊断敏感性为93%-86%，诊断特异性为95%-100%。因此，通过DIG-ELISA法测定麦醇溶蛋白抗体可作为乳糜泻检测试验以及小肠活检患者选择的方法。