Hahn G
Zentralbl Bakteriol Mikrobiol Hyg A. 1983 Dec;256(2):138-45.
Intoxications by S. aureus enterotoxins are predominant in our country, together with enteric infections by Salmonella spp. For lack of a specific therapy the possibility was examined to neutralize orally provided toxin in vivo. For this purpose before and after the intoxication, respectively, antitoxin and normal rabbit serum as a control were also applied per os. In extensive trials SPF-mice were used as a model in spite of their insensitivity to staphylococcal toxins even with 10000 times the emetic dose for monkeys. By serological means, the rate of free toxin in stomach, intestinal contents and fecal matter after and before application of antiserum was identified as an indicator for the neutralization of the toxin in vivo. As toxin, S. aureus enterotoxin B (SEB) was used which could be identified in the samples up to three hours after oral feeding. Antiserum was obtained by immunization of rabbits with toxoid. The application of antiserum one hour after intoxication resulted in an average reduction of free toxin in the intestine of 87,5 and 86,5%, (10 independent trials) one and two hours, respectively after serum administration. In contrast, administration of antiserum one hour before experimental intoxication did not produce statistically confirmed differences between the experimental and the control animals.
在我国，金黄色葡萄球菌肠毒素中毒与沙门氏菌属引起的肠道感染一样普遍。由于缺乏特异性治疗方法，研究了在体内中和口服毒素的可能性。为此，分别在中毒前后经口给予抗毒素和作为对照的正常兔血清。尽管无特定病原体（SPF）小鼠对葡萄球菌毒素不敏感，即使给予猴子催吐剂量10000倍的毒素也不敏感，但在广泛试验中仍将其用作模型。通过血清学方法，将抗血清应用前后胃、肠内容物和粪便中游离毒素的比率确定为体内毒素中和的指标。作为毒素，使用了金黄色葡萄球菌肠毒素B（SEB），口服后长达三小时的样本中均可检测到该毒素。通过用类毒素免疫兔子获得抗血清。中毒后一小时应用抗血清，血清给药后一小时和两小时，肠道中游离毒素平均减少87.5%和86.5%（10次独立试验）。相比之下，在实验性中毒前一小时给予抗血清，实验动物和对照动物之间未产生统计学上得到证实的差异。
