Behrens W, Bückmann D
Gen Comp Endocrinol. 1983 Jul;51(1):8-14. doi: 10.1016/0016-6480(83)90091-6.
Two ecdysteroids were isolated from juveniles and adults as well as from eggs of the pycnogonid Pycnogonum litorale Ström and characterized by bioassay, thin layer chromatography, gas liquid chromatography, and high performance liquid chromatography. One of the hormones could be identified as 20-hydroxyecdysone whereas the other behaves in HPLC in a way that is similar to but not identical with ecdysone. This unidentified ecdysone-like hormone exceeded 20-hydroxyecdysone in all developmental stages (1.67:1 to 3:1). Determination of moulting hormone titers by a radioimmunoassay showed highest amounts of hormones in juvenile females (150 ng/mg tissue dry wt). The results are compared with those obtained from other arthropods and are discussed in view of the evolution of hormonal systems.
从海蜘蛛Pycnogonum litorale Ström的幼体、成体以及卵中分离出了两种蜕皮甾体，并通过生物测定、薄层色谱、气液色谱和高效液相色谱对其进行了表征。其中一种激素可鉴定为20-羟基蜕皮酮，而另一种在高效液相色谱中的行为与蜕皮酮相似但不完全相同。这种未鉴定的类蜕皮酮激素在所有发育阶段的含量都超过了20-羟基蜕皮酮（比例为1.67:1至3:1）。通过放射免疫测定法测定蜕皮激素滴度，结果显示幼年雌性体内的激素含量最高（150 ng/mg组织干重）。将这些结果与从其他节肢动物获得的结果进行了比较，并从激素系统的进化角度进行了讨论。
